产品名称:台盼蓝 溶液(0.4%, liquid, sterile-filtered, suitable for cell culture)
别名:Blue cell viability dye
货号:T8154
CAS号:72-57-1
属性
无菌性:sterile-filtered
质量水平:200
形式:liquid
储存条件:dry at room temperature
浓度:0.4%
technique(s):cell culture | mammalian: suitable
tissue processing: suitable
application(s):cell analysis
运输:ambient
SMILES string:
[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI:
1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChI key:GLNADSQYFUSGOU-UHFFFAOYSA-J
说明
1、一般描述
台盼蓝是一种用于区分活细胞和死细胞的染料。它是一种不会被健康的活细胞所吸收但会对细胞膜受损的细胞进行染色的重要染料。[1]这样,仅有死细胞会被计数。这种方法有时也被称为染料排除方法。
Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue.[2] It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.[3]
2、应用
Trypan Blue solution has been used:
(1)in cell viability assay to count viable and dead cells[4]
(2)in rescue assay to count viable cells[4]
(3)in trypan blue exclusion counting method to determine proliferation curve[5]
(4)as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion[6]
(5)in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability[7]
(6)to count GEC cells using a hemocytometer[7]
3、包装:20, 100 mL
4、制备说明
在 0.81% 氯化钠 和 0.06% 磷酸氢二钾中配制。
选型
SKU 包装规格
T8154-20ML 20 ML
T8154-100ML 100 ML
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